Fig. 7: Disrupted neuronal connectivity in Kcnb1^R312H(+/+) mice.

A Representative z-stack reconstructions (N = 20 sections) of corticocortical and corticothalamic connections in a WT and Kcnb1^R312H(+/+) brain visualized with antegrade BDA 488 MW3000 injected into the neocortex. Scale bars 200 μm. B Representative z-stack magnifications of the images in (A) visualizing the indicated brain structures (CC corpus callosum; IPS CX to CC efferent ipsilateral cortical fibers in the CC, CNT CX contralateral cortex—specular to the site of injection of the tracer, Cp internal capsule, Tha thalamus). Scale bars 50 μm. C Connectivity z-stack (30 images) maps for WT and Kcnb1^R312H(+/+) were drawn from experiments with BDA 488 MW3000. Colors illustrate the density of projections (in %) ranging from green (66–100%) to bronze (33–65%) to red (0–32%). The blue color is the site of the injection. N = 4 brains/genotype. Scale bars 0.2 cm. D Quantifications of connectivity assessed with BDA 488 MW3000 in the ipsilateral cortex (IPS CX), contralateral cortex (CNT CX), Thalamus (Tha), and CC in WT and Kcnb1^R312H(+/+) mice. BDA MW3000 Alexa 488, was injected into the cortex at the following coordinates in mm: Mid: − 1.2, Bregma: −0.6, Top: −1.0. Images were taken from three-month-old brain slices cut at the following coordinates in mm: CC Mid = 0; Bregma = −0.6, Top = −1.2. IPS CX to CC Mid = −1.2; Bregma = −0.6 and Top = −1.0. CNT CX: Mid = 1.2, Bregma = −0.6 and Top = −1.0. Cp: Mid = −2.0, Bregma = −1.06/−1.22 and Top = −2.5/-3.0. Tha: Mid = −1.8, Bregma = −1.34/−1.46 and Top = −3.0. ^*P < 0.05 and ^**P < 0.01, two-tailed student’s t-test.