Fig. 9: The IKC modulates actin polymerization through integrins.
A FRAP in WT dendrites in the absence (Cnt, dotted line, N = 28 cells) or presence of 50 μg/mL fibronectin (FN, squares, N = 18 cells) or 50 μg/mL FN + 100 nM GxTx (FN + GxTX, triangles, N = 26 cells) applied 15 min before FRAP. B As in (A) for Kcnb1R312H(+/+) dendrites. NCnt = 30 cells; NFN = 20 cells; and NFN+GxTx = 21 cells. C FRAP in WT dendrites in the absence (Cnt. dotted line, N = 28 cells) or presence of 30 mM [K]o ([K], squares, N = 14 cells) or 30 mM [K]o + 100 nM GxTx ([K] + GxTx, triangles, N = 27) applied 5 min before FRAP. D As in (C) for Kcnb1R312H(+/+) neurons. NCnt = 30 cells; NK+ = 18 cells and NK+GxTx = 22 cells. E FRAP in WT and Kcnb1R312H(+/+) dendrites in the presence of 100 nM Cilengitide (Cil, WT circles, N = 19 cells; R312H triangles, N = 13) or 10 nM PND-1186 (PND, WT squares, N = 22; R312H triangles, N = 10) applied 15 min before FRAP. F Mobile fraction (fluorescence recovery at time =10 s) for WT neurons subjected to the indicated experimental conditions. G Mobile fraction for Kcnb1R312H(+/+) neurons subjected to the indicated experimental conditions. All measurements were taken on DIV14 neurons. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 one-way ANOVA with Tukey’s post hoc test.
