Fig. 2: Low levels of hepsin result in increased liver size in adult mice by inducing hepatocyte proliferation while maintaining normal liver function. | Communications Biology

Fig. 2: Low levels of hepsin result in increased liver size in adult mice by inducing hepatocyte proliferation while maintaining normal liver function.

From: Enhancement of adult liver regeneration in mice through the hepsin-mediated epidermal growth factor receptor signaling pathway

Fig. 2

a Levels of human hepsin in serum samples on Days 4 and 14 in mice after administering a low dose of AAV-hHPNWT, AAV-hHPNRS, or AAV-EGFP (control AAV). b The ratio of liver to body weight were determined on Day 14. c The correlation between the liver-to-body weight ratio and serum human hepsin levels on Day 14 following AAV administration at a dosage of 1 × 10¹² vg/mouse (black), 1 × 10¹¹ vg/mouse (red), and 2.5 × 10¹⁰ vg/mouse (blue). d Immunoblotting analysis of cyclin D1 on Day 14. e, f Serum alanine aminotransferase (ALT) and total bilirubin (BIL-T) levels on Days 4 and 14. At least n = 5 biologically independent animals were used per group. Dots represent values for individual mice, and red lines represent group means ± SD. Statistical significance was assessed using two-way ANOVA with Sidak’s post-test at each time point in (a, b, e, f). Spearman correlation analysis was used in (c). Significance levels are denoted by asterisks as follows: ***p < 0.001, ****p < 0.0001.

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