Fig. 3: Expression pattern of the liver transcriptome after overexpression of human hepsin.

a Principal component (PC) analysis plots depicting the integrated gene expression data matrix for mouse liver on Days 1, 2, and 4 post-administration of AAV-EGFP (control AAV) or AAV-hHPN^WT. b Venn diagram illustrating the overlap and differential expression of transcripts on Days 1, 2, and 4 between the AAV-EGFP (control AAV) and AAV-hHPN^WT groups. c Unsupervised clustering analysis of the AAV-EGFP (control AAV) and AAV-hHPN^WT groups based on the common expressed transcripts across Days 1, 2, and 4. d, e Clustering analysis of transcripts associated with metabolism and cell proliferation from the shared expressed transcripts on Days 1, 2, and 4. f, g Results of Gene Set Enrichment Analysis (GSEA) in the AAV-hHPN^WT and AAV-EGFP groups on Days 1 and 4, respectively. Each group consists of three mice. For the clustering analysis to generate a heatmap, each column corresponds to a specific time point, and the values within each column represent the average probe intensity of the transcriptomes obtained from three individual mice.