Fig. 5: Effect of an EGFR inhibitor on hepatocyte proliferation induced by hepsin overexpression in adult mice. | Communications Biology

Fig. 5: Effect of an EGFR inhibitor on hepatocyte proliferation induced by hepsin overexpression in adult mice.

From: Enhancement of adult liver regeneration in mice through the hepsin-mediated epidermal growth factor receptor signaling pathway

Fig. 5

a Study design: The irreversible inhibitor of EGFR (CI-1003) was administered daily starting 2 days before the administration of AAV-hHPNWT, AAV-hHPNRS, or AAV-EGFP (control AAV). Immunoblot analysis and quantification of mouse liver lysates were performed on Day 2 after AAV administration. b, c Levels of phosphorylated EGFR (p-EGFR; Tyr1068 and Tyr1148) and total EGFR. d, e Levels of p-c-Raf (Ser289/Ser296/Ser301), total c-Raf, and cyclin D1. f Ratio of liver weight to body weight. At least n = 3 biologically independent animals were used per group. Dots represent individual mouse values, and red lines represent group means ± SD. Statistical significance was assessed using two-way ANOVA with Tukey’s post-test. Significance levels are denoted by asterisks as follows: **p < 0.01, ***p < 0.001, ****p < 0.0001.

Back to article page