Fig. 4: IGF-1 c.258 A > G synonymous mutation inhibits bone resorption and increases bone mass in SOP mice.
From: IGF-1 c.258 A > G synonymous mutation ameliorates senile osteoporosis
a, b The expression level of IGF-1 and IGF-1R in the tibial bone of WT(AA) and Ho(GG) mice at different developmental stages by qRT-PCR. c–g The expression level of RANKL, TRAP, ALP, OCN, OPN, COL-1, OSX, and RUNX2 in tibial bone from different developmental stages WT(AA) and Ho(GG) mice was analyzed using qRT-PCR. h–m Levels of ALP, P1NP, CTX-1, OPG, and RANKL were measured in serum of different developmental stages of WT(AA) and Ho(GG) mice using ELISA. OPG, osteoprotegerin; P1NP, procollagen type 1 N-terminal propeptide; CTX C-terminal telopeptide of type I collagen, RANKL receptor activator of NF-κB ligand. All data shown were obtained from male mice. The results are presented as mean ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001.
