Fig. 6: IGF-1 c.258 A > G synonymous mutation promotes bone formation in an osteoblast-osteoclast co-culture system.
From: IGF-1 c.258 A > G synonymous mutation ameliorates senile osteoporosis
a–e The expression level of osteogenesis and osteoclast-related genes in H2O2-induced primary osteoblasts and osteoclast with two genotypes in co-culture by qRT-PCR. f–h The expression level of OPG and RANKL in aged primary osteoblasts and osteoclast with two genotypes in culture alone and co-culture by ELISA. i–m The expression level of osteogenesis and osteoclast-related genes in aged MC3T3-E1 with two genotypes and RAW264.7 in co-culture by qRT-PCR. n–p The expression level of OPG and RANKL in aged MC3T3-E1 with two genotypes and RAW264.7 in co-culture by ELISA. The results are presented as mean ± SD (n = 5). *p < 0.05; **p < 0.01; ***p < 0.001.
