Fig. 1: Characteristics of the Paraburkholderia phymatum STM815^T transposon sequencing (Tn-seq) library.

a P. phymatum STM815^T essential genes distributed among the two chromosomes and two plasmids. From the outside in, the backbone of each replicon, the plus strand of CDS, the minus strand of CDS and location of essential genes (showed in blue). The segment brackets are displayed at 100 kb intervals. Image performed with Clico FS¹¹². b Percentage of essential genes belonging to each COG (Cluster of Orthologous Genes) functional category^106,113. Genes were annotated with eggNOG mapper v2^107,108. Asterisks (*) indicate statistical significance of over-represented genes in a specific category (p-value < 0.01). The number of essential genes belonging to each COG category are annotated on top of its corresponding bar. B: Chromatin Structure and dynamics; C: energy production and conversion; D: cell cycle control and mitosis; E: amino acid metabolism and transport; F: nucleotide metabolism and transport; G: carbohydrate metabolism and transport; H: coenzyme metabolism; I: lipid metabolism; J: translation, ribosomal structure, and biogenesis; K: transcription; L: replication, recombination, and repair; M: cell wall, membrane, and envelop biogenesis; N: cell motility; O: post-translational modification, protein turnover, and chaperon; P: inorganic ion transport and metabolism; Q: secondary structure; S: function unknown; T: signal transduction mechanisms; U: intracellular trafficking, secretion, and vesicular transport; V: defense mechanisms; W: extracellular structures.