Fig. 2: Identification of P. phymatum Tn mutants with nodulation abilities in soybean. | Communications Biology

Fig. 2: Identification of P. phymatum Tn mutants with nodulation abilities in soybean.

From: Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean

Fig. 2

a Methodology used to inoculate and process the P. phymatum Tn-seq library on soybean seedlings. After 21 days, nodules containing Tn-seq mutants were collected, DNA from the nodules was extracted, and libraries were generated for sequencing using the circularization method. The obtained reads were mapped to the genome of P. phymatum STM815T and the exact position of the transposon (Tn) in the genome was determined. This figure was created anew by the authors using PowerPoint 2013 (Microsoft 365). b Soybean plants inoculated with B. diazoefficiens USDA 110Tspc4 (positive control), P. phymatum STM815T wild-type (negative control), and seven soybean plants inoculated with P. phymatum Tn-seq library with developed nodules (Tn-seq plants). Scale bar: 2 mm.

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