Fig. 5: The P. phymatum nodS mutant colonizes intracellularly the central zone cells of soybean nodules. | Communications Biology

Fig. 5: The P. phymatum nodS mutant colonizes intracellularly the central zone cells of soybean nodules.

From: Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean

Fig. 5

a Representative soybean root nodules induced by B. diazoefficiens USDA 110Tspc4 and (b) by the P. phymatum nodS mutant. c After cutting open the nodules in (a) and (b) the interior of (a) suggested a higher leghemoglobin content (red area in the nodule centre) in the B. diazoefficiens-colonized nodules (c) compared to those colonized by the P. phymatum nodS mutant (d). e Light micrograph of a transverse section of a nodule formed by B. diazoefficiens USDA 110Tspc4 and (f) of a nodule formed by the P. phymatum nodS mutant. g Semi-thin section of the nodule in e. h Semi-thin section of the nodule in f. i TEM of a nodule formed by B. diazoefficiens USDA 110Tspc4 and j of a nodule formed by the P. phymatum nodS mutant showing bacteroids being degraded (indicated with a black arrow) and lytic vacuoles enlarging (*). Scale bar (a–f): 500 µm; (g, h): 10 µm; (i, j): 500 nm.

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