Fig. 3: A heatmap visualization of the sensitivity of metabolic reactions to a specific EMT stage.

The sensitivity of the reactions is determined by the reduction in the normalized KO Biomass flux, referred as Sensitivity Score. At any timepoint, the reactions showing a sensitivity score closer to a value of −1 have the highest sensitivity as compared to simulated reaction knockout in the control (an unconstrained metabolic reconstruction growth rate biomass flux upon reaction knockout in an unconstrained model, see “Methods”). Reactions are sorted based on prioritization scores. The bar plots (inset) show the distribution of sensitive reactions (sensitivity score < 0) for three timepoints 1 h, 24 h, and 48–72 h after TGF-β induction across all experiments in 3 time points. The red color in the heatmap represents a reduction in the Biomass flux upon reaction knockout, as compared to the control (sensitivity score < 0). The gray to dark gray colors represents an increase in the Biomass flux (sensitivity score > 0).