Fig. 4: siRNA-mediated inhibition of Eno3, but not Eno1, triggers apoptosis in A549 cells undergoing EMT after 72 h TGF-β treatment. | Communications Biology

Fig. 4: siRNA-mediated inhibition of Eno3, but not Eno1, triggers apoptosis in A549 cells undergoing EMT after 72 h TGF-β treatment.

From: Genome-scale modeling identifies dynamic metabolic vulnerabilities during the epithelial to mesenchymal transition

Fig. 4

“Control” indicates no siRNA, and “Scrm” indicates scrambled siRNA. Efficiency of siRNA mediated knockdown of Eno1 and Eno3 was assessed by western immunoblotting and qRT-PCR (Supplementary Fig. 6A, B). A Apoptosis is assessed by staining for the percentage of AnnexinV/PI +ve cells by Flow cytometry (a representative gating strategy for 4 samples is presented in Supplementary Fig. 8). Histogram represent mean of three replicates with error bars representing standard error. Statistical significance assessed by paired t test * = <0.05, ** = <0.005. B Caspase3/7 activation is measured using a caspase3/7 specific substrate that fluoresces red after caspase3/7-mediated cleavage, cells were imaged and the number of red fluorescent cells per 96-well were counted (Supplementary Fig. 6C) using the CellCyte (Echo, San Diego, CA), a live cell imaging system. Data is representative of three independent biological replicates.

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