Fig. 2: CA2-DRD regulates the abundance of different classes of protein in response to ACZ exposure.

a Construct schematic of N-terminal CA2-DRD or WT CA2 fused to full-length c-Jun, with mCherry as a transduction marker. b Western blot showing detection of c-Jun and phosphorylated c-Jun in presence of DMSO or ACZ in Jurkat cells transduced with constructs 001 or 002. Mock is lysate from empty vector-transduced Jurkat cells. c Quantification of the Western blot shown in (b). d Construct schematic of CA2-DRD or WT CA2 fused to aflibercept. e ELISA measurement of secreted aflibercept in the presence of ACZ or DMSO from transfected HEK cells after 24 h of cell culture. Mock sample is supernatant from HEK cells transiently transfected with empty vector. Mock and CA2-wt (constitutive aflibercept) response to ACZ were not measured. Error bars show mean with range (N = 2). f Construct schematic (labeled as V1) of CA2-DRD–regulated membrane-bound cytokines expressed in Jurkat cells, whose ACZ dose-responses are shown in g (IL15) and h (IL12). Error bars represent standard deviation (N = 3). P-values are from a paired two-tailed T-Test. The experiments were repeated at least two times with similar results.