Fig. 3: Membrane-bound scaffolds that promote multimerization can effectively regulate IL12 abundance. | Communications Biology

Fig. 3: Membrane-bound scaffolds that promote multimerization can effectively regulate IL12 abundance.

From: Carbonic anhydrase 2-derived drug-responsive domain regulates membrane-bound cytokine expression and function in engineered T cells

Fig. 3

Schematic of designed membrane-bound IL12 constructs using multimerizing domains from (a) collectin-7 and (b) collagen 18. c Putative intracellular assembly of the designed construct shown in (a). d Putative intracellular assembly of the designed construct shown in (b). e and f Detection of IL12 on Jurkat cell surface in the presence or absence of ACZ. g Schematic showing intracellular repositioning of the collectin-7 trimerizing domain. h Schematic showing multimerization of IL12 through its TM. i Bar graph showing IL12 surface expression levels on T cells in the presence or absence of ACZ for the construct with intracellularly repositioned trimerizing domains as shown in (g) and (h) and compared with construct format V1. Error bars represent standard deviation (N = 3). P-values are from a paired two-tailed T-Test. The experiments were repeated at least three times with similar results.

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