Fig. 4: Optimized modulation hubs can be used to regulate cell surface expression of a broad range of cytokines. | Communications Biology

Fig. 4: Optimized modulation hubs can be used to regulate cell surface expression of a broad range of cytokines.

From: Carbonic anhydrase 2-derived drug-responsive domain regulates membrane-bound cytokine expression and function in engineered T cells

Fig. 4

a Schematics of representative constructs (V1–V6) that were used to regulate the surface expression of b IFNα, c IL23 and d IL2 in Jurkat cells. The construct version (V1–V6) used for each of the cytokines is indicated on the x-axis labels. In each schematic the domain responsible for the multimerization is in green, the cargo in black, the DRD in blue and linkers and hinges are highlighted in orange. Error bars represent standard deviation (N = 3). P-values are from a paired two-tailed T-Test. The experiments were repeated at least three times with similar results.

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