Table 1 Flow cytometry reagents
Antigen | Fluorophore | Vendor | Catalogue Number | Dilution |
|---|---|---|---|---|
IL12 P40 | V-450 | BD | 561380 | 1:100 |
IL12 P40 | PE | BioLegend | 501807 | 1:100 |
IL2 | APC | BioLegend | 500311 | 1:100 |
IFNa | PE | BD | 560097 | 1:100 |
IL1b | FITC | BioLegend | 511705 | 1:100 |
IL18/IL1F4 | Unconjugated - Mouse IgG | Novus | 925008 | 1:100 |
Anti-Mouse IgG | AF647 | Jackson Immunoresearch | 115-605-071 | |
IL21 | APC | ThermoFisher | 17-7211-82 | 1:100 |
Live/Dead Dye | NIR | eBioscience | 65-0865-14 | 1:100 |
Human FC-Block | Unconjugated | BioLegend | 422302 | 1:100 |
CD56 | FITC | BioLegend | 318304 | 1:100 |
CD3 | PE-Cy7 | BioLegend | 344816 | 1:100 |
pSTAT4 | PE | BD | 562074 | 1:20 |
IL12 P70 | PE | BD | 559325 | 1:100 |
CD19-Fc | Unconjugated, Human Fc fusion | R&D Systems | 9269-CD | 1:200 |
CD34/RQR8 | APC | R&D Systems | FAB7227A | 1:100 |
Thy1.2 | BV421 | BioLfegend | 105341 | 1:100 |
Anti-Human IgG | BV421 | BioLegend | 409318 | 1:200 |
FACS buffer | BD | 420201 | ||
Counting Beads | BioLegend | 424902 |
