Fig. 1: Microglial SMEK1 expression fluctuated with time after MCAO/R and OGD/R.

A Schematic diagram of the mouse experimental design. B Schematic diagram of the sampling sites. C Schematic diagram of the experimental design for BV2 cells. Determination of SMEK1 mRNA (D) and protein (E) expression in the Sham and MCAO/R 1 d, 3 d and 7 d groups. n = 3 per group. F Representative photos of SMEK1⁺ cells in the Sham, MCAO/R 3 d and Contralateral groups, scale bar: 100 μm. G Representative photos of SMEK1⁺ microglia in the Sham and MCAO/R 3 d groups, scale bar: 100 μm. H Gating strategy for SMEK1⁺ microglia isolated from the brain after MCAO/R and the proportion of SMEK1⁺ microglia in the MCAO/R 3 d and Contralateral groups. n = 8 per group. SMEK1 mRNA (I) and protein (J) expression in BV2 cells subjected to OGD for 3 h followed by exposure to a normal oxygen concentration for 0, 1, 6, 12, 21 or 24 h. n = 3 per group. K Representative photos of SMEK1⁺ BV2 cells in the control and OGD/R21h groups, scale bar: 100 μm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. Sham or Control group. ns not significant.