Fig. 7: Microglial SMEK1 deficiency promotes histone lactylation via the PDK3-PDH signaling pathway. | Communications Biology

Fig. 7: Microglial SMEK1 deficiency promotes histone lactylation via the PDK3-PDH signaling pathway.

From: Therapeutic potential of microglial SMEK1 in regulating H3K9 lactylation in cerebral ischemia-reperfusion

Fig. 7

A Determination of PDK3 mRNA expression in shNC, shSMEK1, oeNC and oe-SMEK1 BV2 cells. n = 3 for each group. B PDH activity of shNC, shSEMK1, oeNC and oe-SMEK1 BV2 cells. n = 4 per group. C The protein expression of PDK3, PDH and P-PDH in shNC, shSMEK1, oeNC and oe-SMEK1 BV2 cells was assessed using WB. Extracellular (D) (n = 3 per group) and intracellular (E) (n = 4 per group) LA levels in shNC, shSMEK1, oeNC and oe-SMEK1 BV2 cells. F Determination of Pan-Kla, H2BK16la, H3K9la, H3K14la, H3K18la, H4K5la, H4K8la, H4K12la and H4K16la protein expression in control, OGD/R21h BV2 cells and Sham, MCAO/R 3 d mice. n = 3 for each group. G Representative photos of H3K9la+ microglia in the Sham and MCAO/R 3 d groups, scale bar: 50 μm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. shNC or oeNC or Control or Sham group. ns not significant.

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