Fig. 5: Deletion of Brd4 enhances gut barrier integrity in HFD-fed mice. | Communications Biology

Fig. 5: Deletion of Brd4 enhances gut barrier integrity in HFD-fed mice.

From: Brd4 modulates metabolic endotoxemia-induced inflammation by regulating colonic macrophage infiltration in high-fat diet-fed mice

Fig. 5

A Plasma levels of FD4 in WT or Brd4-CKO mice fed with ND or HFD for 4 weeks (left) or 20 weeks (right) were measured (n = 5–7). B mRNA levels of Occludin, Claudin-1, and Zo-1 in the colon of WT or Brd4-CKO mice fed with ND or HFD for 6 weeks were quantified (n = 5). C Protein levels of Occludin and Claudin-1 in the colon of WT or Brd4-CKO mice fed with ND or HFD for 6 weeks were assessed. (D&E) ELISA was conducted to determine the levels of lipopolysaccharide (LPS) in the plasma (D) or eWAT (E) of WT or Brd4-CKO mice fed with ND or HFD for 6 weeks (n = 5). Data are mean and SD and are determined by an unpaired 2-tailed Student’s t-test (A, right panel) or 1-way ANOVA (A, left panel; B; D&E). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns, statistically not significant.

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