Fig. 6: Effect on cell type- and domain-specific activation in the OT of local injection of an OxR1 antagonist into the amOT. | Communications Biology

Fig. 6: Effect on cell type- and domain-specific activation in the OT of local injection of an OxR1 antagonist into the amOT.

From: Function of orexin-1 receptor signaling in the olfactory tubercle in odor-guided attraction and aversion

Fig. 6

a c-Fos(+) cells in the OT of vehicle-injected (left) and antagonist-injected (right) mice. Red, c-Fos; blue, DAPI. D, dorsal; V, ventral; M, medial; L, lateral. Scale bar, 500 μm. b c-Fos(+) cell density in the amOT and lOT of vehicle-injected (white columns) and antagonist-injected (black columns) mice. c c-Fos/D1 and c-Fos/D2 double-positive cells in the amOT and lOT of vehicle-injected and antagonist-injected mice. (Leftmost column) c-Fos/D1 co-detection in the amOT of a vehicle-injected mouse. (Second-left column) c-Fos/D2 co-detection in the amOT of a vehicle-injected mouse. (Second-right column) c-Fos/D2 co-detection in the amOT of an antagonist-injected mouse. (Rightmost column) c-Fos/D1 co-detection in the lOT of an antagonist-injected mouse. Red, c-Fos mRNA; green, D1/D2 mRNA. Double-positive cells (arrows) and c-Fos-single-positive cells (arrowheads) are indicated. Scale bar, 10 μm. d Densities of c-Fos/D1 and c-Fos/D2 double-positive cells in the amOT and lOT of vehicle-injected (white columns) and antagonist-injected (black columns) mice. e Ratios of c-Fos/D1 cell density versus c-Fos/D2 cell density in the amOT of vehicle-injected (white column) and antagonist-injected (black column) mice. A dotted line indicates the ratio at 1. f Ratios of c-Fos/D1 cell density versus c-Fos/D2 cell density in the lOT of vehicle-injected (white column) and antagonist-injected (black column) mice. A dotted line indicates the ratio at 1. b, d, e, f Average ± SD; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001. b, d n = 6 from left/right OTs of 3 mice for vehicle-injected mice, n = 8 from left/right OTs of 4 mice for antagonist-injected mice (one-way ANOVA with Tukey’s multiple comparison test). e, f Each dot represents a ratio within each mouse; 3 vehicle-injected mice, 4 antagonist-injected mice (t-test).

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