Table 1 Summary of capture statistics for each protocol

From: Optimized methods for scRNA-seq and snRNA-seq of skeletal muscle stored in nucleic acid stabilizing preservative

Sample

Preparation

Captured

No. samples after gene level-based filtration*

(% of captured)

No. samples after simulated doublet detection**

(% of UMI-filtered)

Cells

Filtered

48,802

18,805 (39%)

17,958 (95%)

Nuclei

Filtered

24,598

19,977 (81%)

18,704 (94%)

Cells

Sorted

3470

2031 (59%)

2028 (99%)

Nuclei

Sorted

3995

1936 (48%)

1922 (99%)

  1. * UMI-filtration excluded cells with ≤200 genes, ≥2500 genes, or >10% reads mapped to mitochondrial RNAs.
  2. ** Simulated doublet detection and exclusion was performed with DoubletFinder24.
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