Fig. 1: Structure and CMC-based detection of Ψ in RNA.
From: Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase
a Chemical structures showing the formation of Ψ upon the isomerization of U. b Reaction scheme of CMC with Ψ yielding the N3 CMC-Ψ adduct (“CMC-Ψ”). c Illustrations of RT stop and read-through events against the CMC-Ψ.
