Fig. 3: Characterization of Ψ into CMC-Ψ conversion and RNA loss by varying the duration of CMC reaction.
From: Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase
a Shown is the increasing Ψ into CMC-Ψ conversion efficiency of Ψ-oligo1 with the elongated CMC reaction duration, measured by the RT stop assay. b Dependence of the Ψ into CMC-Ψ conversion of Ψ-oligo1 RNA and the RNA recovery after CMC treatment, upon the CMC reaction duration.
