Fig. 4: Promoted mutation signatures against CMC-Ψ in rRNAs by RT-1306 in “piloting” Ψ-seq libraries.
From: Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase
a Representative IGV views showing RT mutations at two example Ψ sites in 18S and 28S rRNAs. b Ψ1177 in 18S rRNA with 20-min or 2-h CMC reaction. c Profiling of RT mutation, stop and deletion signatures at all reported Ψ sites in rRNAs by RT-1306, with statistical comparisons to wtRT and SSIII. d Profiling of RT signatures by RT-1306 at Ψ sites in rRNAs detected with 20-min and 2-h CMC reaction. P values were calculated by two-sided Student’s t-test, with the significance levels noted where ns. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
