Fig. 5: Development and results of Mut-Ψ-seq.
From: Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase
a Workflow and list of prepared libraries for Mut-Ψ-seq. b Comparison of Ψ identification efficiency by individual or combined RT signatures generated by RT-1306 or SSIII, through the AUC values derived from ROC curve analyses against reported Ψs and Us in rRNAs (Supplementary Fig. 9 and Supplementary Fig. 10). c Shown on the left is the ROC analyses for assessing Ψ identification efficiency of RT-1306 by 20-min or 2-h CMC reaction. Shown on the right are the distributions of observed combined rates generated by RT-1306 against reported 1088 Us (upper panel) and 105 Ψs (low panel) in rRNAs. P values were calculated by two-sided Student’s t-test, with the significance levels noted where ****P < 0.0001. d Representative IGV views of the RT mutation signature suggesting the occurrence Ψ in GUΨC sequence in four mRNAs.
