Table 1 High-confidence Ψ sites identified by both Mut-Ψ-seq and PRAISE

From: Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase

Refseq

Name

Region

Sequence motif

NM_020992.4:317

PDZ and LIM domain 1

CDS

CUUG

NM_023934.4:234

FUN14 domain containing 2

CDS

CUUUUG**

NM_000983.4:313

Ribosomal protein L22

CDS

GUG

NM_014014.5:62

Small nuclear ribonucleoprotein U5 subunit 200

5′UTR

CUUG

NM_001253384.2:631

Ribosomal protein L15

CDS

GUA

NM_000980.4:247

Ribosomal protein L18a

CDS

GUG

NM_018457.4:148

Proline rich 13

CDS

GUUC*

NM_012423.4:456

Ribosomal protein L13a

CDS

GUUG**

NM_207346.3:305

tRNA splicing endonuclease subunit 54

CDS

GUUG*

NM_213611.3:887

Solute carrier family 25 member 3

CDS

CUUUG**

NM_052844.4:399

Dynein 2 intermediate chain 2

CDS

GUG

NM_003302.3:1434

Thyroid hormone receptor interactor 6

CDS

CUG

NM_033251.2:823

Ribosomal protein L13

CDS

CUUC**

NM_002080.4:241

Glutamic-oxaloacetic transaminase 2

CDS

CUUUA

NM_004077.3:672

Citrate synthase

CDS

CUUUG

NM_017952.6:495

Pentatricopeptide repeat domain 3

CDS

AUUG

NM_001319140.2:639

Adenylate kinase 2

CDS

GUUC*

NM_012111.3:465

Activator of HSP90 ATPase activity 1

CDS

CUUG

NM_001199973.2:242

RPL36A-HNRNPH2 readthrough

CDS

CUUG

NM_015975.5:2436

TATA-box binding protein associated factor 9b

3′UTR

GUUC*

NM_001142285.2:92

Ribosomal protein S24

CDS

CUUC**

NM_006816.3:778

Lectin, mannose binding 2

CDS

CUUC**

NM_001199629.2:594

Myosin light chain 6B

CDS

CUUG**

NM_019059.5:228

Translocase of outer mitochondrial membrane 7

3′UTR

CUUUG**

NM_000990.5:179

Ribosomal protein L27a

CDS

CUUUG**

NM_001679.4:293

ATPase Na+/K+ transporting subunit beta 3

CDS

GUUUUUUA**

NM_003795.6:296

Sorting nexin 3

CDS

AUUUUC**

NM_012268.4:1692

Phospholipase D family member 3

CDS

CUG

NM_001199111.2:699

Malate dehydrogenase 1

CDS

CUUG**

NM_053275.4:748

Ribosomal protein lateral stalk subunit P0

CDS

CUUG**

NM_198552.3:779

Family with sequence similarity 89 member A

3′UTR

CUUUG

NM_004450.3:648

ERH mRNA splicing and mitosis factor

3′UTR

GUUC*

NM_001402.6:519

Eukaryotic translation elongation factor 1 alpha 1

CDS

GUUA

NM_014394.3:166

Growth hormone inducible transmembrane protein

CDS

CUA

NR_104080.1:85

U6 small nuclear 9

AUUCG

NR_001445.2:247

7SK nuclear ribonucleoprotein

AUUUG

NR_001445.2:250

7SK nuclear ribonucleoprotein

GUA

NR_104080.1:40

U6 small nuclear 9

AUA

NR_002569.2:69

Small Cajal body-specific RNA 9

CUUUC**

NR_003932.2:457

Ribosomal protein L13a pseudogene 20

GUUG

  1. Ψs identified by the reported criteria in Mut-Ψ-seq are underlined; when Ψ is detected in UU sequence contexts, the nearby U positions with significant mutation signature which suggests the most probable position of Ψ, are colored in red.
  2. * or ** denotes potential substrates for TruB1 or DKC1, respectively, reported by knockdown studies by PRAISE25.