Fig. 1: IGF2BP1 was identified and verified as a new citrullinated protein in RA-FLSs. | Communications Biology

Fig. 1: IGF2BP1 was identified and verified as a new citrullinated protein in RA-FLSs.

From: Citrullinated IGF2BP1 promotes rheumatoid synovial aggression via increasing the mRNA stability of SEMA3D

Fig. 1

A The global citrullination level of proteins in FLSs from HC (n = 4) and RA (n = 4) were analyzed by western blot. B MS1 chromatograms of citrullinated proteins from RA-FLSs. C The MS2 fragmentation spectra of IGF2BP1 indicating the citrullination site of R167. A mass increase of 0.98 Da, together with neutral loss of NHCO (43 Da) and NH3 (17 Da) were marked in red, which are diagnostic criterions for protein citrullination. D Citrullination levels of IGF2BP1 in FLSs from HC (n = 9) and RA (n = 12) were detected by western blot using F95 antibody. E Citrullination levels of IGF2BP1 in RA-FLSs from ACPA+ (n = 8) and ACPA− (n = 4) patients were detected by western blot using F95 antibody. F The citrullination level of recombinant human IGF2BP1 was detected by western blot. G Schematic illustration of the citrullination sites of IGF2BP1 identified by Mass spectrometry. All the reactions were performed in triplicate. The data were presented as mean ± SD. Unpaired t-test (A, D, E) was used as parametric test. *P < 0.05, **P < 0.01.

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