Fig. 3: Cit-IGF2BP1 promoted SEMA3D expression by maintaining its mRNA stability.
A Volcano plots of differently expressed genes (DEGs) between IGF2BP1-KD RA-FLSs rescued with IGF2BP1WT and IGF2BP1R167K (n = 3 per group). B The enriched Gene Ontology (GO) analysis of the DEGs. C The enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the DEGs. D The screening of mRNAs interacting with IGF2BP1 by the ENCORI, RM2Target, and RNA INTER database, three databases that supported by large-scale CLIP-Seq for exploring protein-RNA interactions. E The overlapping of the mRNAs that interacted with IGF2BP1 and the DEGs of the RNA-seq data from this experiment. F, G The verification of SEMA3D as a target gene of cit-IGF2BP1 by qPCR and western blot. H The interaction of IGF2BP1 and SEMA3D mRNA was detected by RIP-qPCR. I The stability of SEMA3D mRNA was detected by q-PCR. Statistical significance was determined by ANOVA of repeated measurements. RA-FLSs were treated with actinomycin D and harvested at 0, 1, 2, 4, and 8 h. All the experiments were performed in triplicate. The data were presented as mean ± SD. Unpaired t-test (F, G, H) was used as parametric test. *P < 0.05, **P < 0.01.
