Fig. 1: Glycosynthase reaction of E350G mutant of Bxy_22780. | Communications Biology

Fig. 1: Glycosynthase reaction of E350G mutant of Bxy_22780.

From: Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium

Fig. 1

a–c Glycosynthase reaction of E350G mutant of Bxy_22780 toward monosaccharides (a), disaccharides (b), and pNP-sugars (c). Asterisks represent the origins of the TLC plates. Reaction time (3 h or overnight) is shown above the bar for each acceptor. “+” and “−” represent that α-GalF was incubated with and without the mutant, respectively. Arrows represent the position of spots of α-GalF. A parenthesis represents that the sample diluted by twice was used. Scales are shown on the right side of the TLC plates to show the position of the spots clearly. a (bottom), The positions of spots derived from galactose and presumed di- and tri-saccharides were indicated as blue and red triangles, respectively, on the left side of the TLC plate. The positions of faint spots derived from glucose and l-rhamnose were indicated by black triangles on the right side of the TLC plates.

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