Fig. 2: Identification of glycosynthase products. | Communications Biology

Fig. 2: Identification of glycosynthase products.

From: Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium

Fig. 2

a Effect of acetone on the synthesis of galactooligosaccharides. (Top) Arrows represent presumed DPs. Asterisks represent the origin of the TLC plate. Percentages represent concentrations of acetone in the reaction solutions. A scale is shown on the right side of the TLC plates to show the position of the spots clearly. (Bottom) A table of production ratio among the products with presumed DP2–4. Color intensities of spots were quantified using the macro prepared by Ohgane et al. 70. b Enlarged view of signals in HMBC representing the glycosidic bond of the galactobiose product. Red and blue labels indicate signals for α and β anomers, respectively. Apostrophes represent non-reducing end galactose units. c The chemical structure of β-1,2-Gal2.

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