Fig. 3: Substrate specificity of Bxy_22780 toward β-galactosides.

Origins of the TLC plates are indicated with an asterisk. Concentrations of substrates used were 5 mM. Concentrations of Bxy_22780 were 0.02 mg/ml. The reaction times (h) were shown above the bold lines and overnight is abbreviated as O/N. Galactose, glucose, and fructose (10 mM each) were used as markers. a Activity toward β-1,2-galactooligosaccharides in 3 h of reaction. b Activity toward galactosides in the reaction overnight.