Table 1 Kinetic parameters of Bxy_22780

From: Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium

 

Vmax (U/mg)

Km (mM)

Vmax/Km (U/mg/mM)

Wild type

pNP-Gal

21.2 ± 1.4

3.49 ± 0.53

6.08 ± 0.58

pNP-d-Fuc

14.0 ± 0.45

7.95 ± 0.46

1.76 ± 0.05

β-1,2-Gal2

14.6 ± 0.3

1.40 ± 0.05

10.4 ± 0.3

β-1,2-Gal3

24.1 ± 0.9

1.65 ± 0.14

14.6 ± 0.8

W288A mutant

β-1,2-Gal2

1.18 ± 0.05

3.22 ± 0.25

0.367 ± 0.015

β-1,2-Gal3

2.08 ± 0.49

54.9 ± 13.7

0.0378 ± 0.0007

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