Fig. 1: Phenotypic analysis of mice with partial deletions of the mXYSRa/Enh13 region for identification of an additional functional sequence.
From: GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse
A Overview of the method used to generate mutant mice. Left half shows in vitro fertilization and genome editing. Pink circle indicates an oocyte. Sperm is shown by the shape. Each genotype is indicated at left. Black and dotted rectangle indicate mXYSRa and deleted mXYSRa, respectively. Fertilized oocytes and mixtures (as a representative case, mixtures for initial screening are shown) for microinjection are depicted at middle. F0 generation mice are shown at right. Deleted sequences are drawn with dotted rectangles. B Schematic diagram of mXYSRa/Enh13 region and the position of responsible sequence. All the XY individuals of the mouse lines listed in this figure had a female-type reproductive system. Genome sequence is shown at top. Bold black box, blue box, green box and red box indicate mXYSRa, NR5A1-BS, SOX9-BS, and SRY-BS, respectively. Positions of gRNAs are shown below the genome sequence with the name. Genotypes of mice are shown below the genome with lines. Black bar: genomic sequence outside of mXYSRa; bold line: mXYSRa; no line: deleted sequence. gRNAs used, deleted sizes are indicated at left with ∆ and parenthesis, respectively. The numbers of XY mice obtained are shown at right. Deleted sequence of mice microinjected gRNA F is shown at bottom with wild-type sequence. Dots indicates deleted bases. Rectangle indicates predicted GATA4 binding sequence.
