Fig. 5: Phenotypic analysis of GATA4-BS2sub/2sub in C57BL/6 J genetic background at adult stage.
From: GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse
A Schematic representation of genomic structure of mXYSRa and Sox9 loci. Primers for genotyping are indicated at top with arrows. Wild-type sequence and substituted are shown at bottom. Substituted sequences are underlined. Black box: mXYSRa; light blue box: NR5A1-BS; green box: SOX9-BS; yellow box: GATA4-BS; red box: SRY-BS; blue box: Sox9; Bold arrow: orientation of transcription. B A result of PCR direct sequence analysis. Electropherogram are shown. Genotypes are shown at top. Wild-type and substituted GATA4-BS sequences are marked with yellow lines. C Phenotypic analysis of XY, GATA4-BS2sub/2sub mice. Genotypes and sex chromosome compositions are indicated at top. a–d External genitals. Arrow heads indicate nipples. e–h Internal genitals. T: testis; O: ovary. The numbers of XY, GATA4-BS2sub/2sub individuals with configuration of gonads among 44 mice are shown at right. i–m HE staining of gonads. Bar: 200 μm. n–r Close up images of region indicated with rectangles in (i–m), respectively. D Box-and-whisker plot of anogenital distance/body weight (AGD/BW) in adult mice. Genotypes, sex chromosome compositions and numbers used for analysis are shown at right. Dots indicates values of each mouse. P-values are shown at the top.
