Fig. 4: The yields of compounds 1–4 in engineered E. coli harboring AscC. Co-transformation pET28a/AscC and pCold-TF empty vector in engineered E. coli containing the pBbA5c-MevT-of MBIS plasmid served as a control for determining the effect of AscC gene copy on product yields. Four biologically independent experiments were performed.

The error bars represent the standard deviation (S.D.) of the mean.