Fig. 5: Analysis of crucial residues in AscC. | Communications Biology

Fig. 5: Analysis of crucial residues in AscC.

From: Engineering a promiscuous prenyltransferase for selective biosynthesis of an undescribed bioactive cannabinoid analog

Fig. 5

A Overview of AscC model docked with FDP and OA. B Interactions between the substrates and individual residues in AscC. Yellow, blue, red, green, orange, violet, and black dash lines represent hydrogen bonds, alkyl interactions, π-alkyl interactions, carbon interactions, charge interactions, π-cation interactions, and donor-donor interactions, respectively. The visualization was generated using Pymol. C Compound yields of wild-type AscC and its mutants expressed in the pET28a vector in engineered E. coli containing the pBbA5c-MevT-MBIS plasmid. **P < 0.01vs WT. Five biologically independent experiments were performed. The error bars represent the standard deviation (S.D.) of the mean. D–G Interactions between the substrates and residues in AscC and its mutant L180F.

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