Fig. 2: Microglial transcriptome analysis at PN3d in the control and MIA groups. | Communications Biology

Fig. 2: Microglial transcriptome analysis at PN3d in the control and MIA groups.

From: Prenatal inflammation impairs early CD11c-positive microglia induction and delays myelination in neurodevelopmental disorders

Fig. 2

A Protocol of microglia isolation and RNA extraction. B Volcano plots of DEGs in microglia isolated from the MIA group compared to the control group at PN3d (n = 3 offspring from 2 to 3 litters in each group). C Heatmap showing the gene expression levels in the microglia isolated from the control and MIA groups at PN3d. D GO enrichment analysis of microglia isolated from the MIA group compared to that of the control group. E qRT-PCR showing the relative expression of several known DEGs associated with microglial function (n = 6 in each group; maximum 2 offspring per litter). Data are presented as means ± SEMs. DEGs differentially expressed genes, MIA maternal immune activation, PN postnatal, SEM standard error of measurement, qRT-PCR quantitative reverse transcription-polymerase chain reaction, GO gene ontology.

Back to article page