Fig. 3: Comparison of myelination-related mRNA and protein. | Communications Biology

Fig. 3: Comparison of myelination-related mRNA and protein.

From: Prenatal inflammation impairs early CD11c-positive microglia induction and delays myelination in neurodevelopmental disorders

Fig. 3

A Protocol of RNA extraction. Mbp and Plp mRNA expression in PN8d offspring between the control and MIA groups (n = 5 in each group; maximum 2 offspring per litter). B MBP and PLP immunostaining of the lateral corpus callosum sections of the control and MIA groups at PN8d (left panel) and PN14d (right panel). Scale bar = 500 µm (lower magnified image) and 200 µm (higher magnified image). C Quantitation results of the percentage of the positive expression area of MBP (upper panel) and PLP (lower panel) in PN8d (n = 5 in each group; maximum 2 offspring per litter) and PN14d offspring (n = 7 in the control group and n = 8 in the MIA group; maximum 2 offspring per litter). D Luxol Fast Blue staining of the lateral corpus callosum of the control and MIA groups at PM14d. Scale bar = 500 µm; Magnified images were cropped into 114 × 68 µm. E Quantitation results of the percentage of the Luxol Fast Blue positively stained area in PN14d offspring (n = 7 in the control group and n = 8 in the MIA group; maximum 2 offspring per litter). Data are presented as means ± SEMs. MIA maternal immune activation, MBP myelin basic protein, PLP proteolipid protein, qRT-PCR quantitative reverse transcription-polymerase chain reaction, LFB Luxol Fast Blue, SEM standard error of measurement.

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