Fig. 5: In silico analysis of CD11c expression.

A Cd11c expression (count per million) of microglia in the whole mouse brain at each stage of development. Each dot represents the average expression from each sample, and the solid line shows the approximate curve by local polynomial regression fitting. These data were generated using the normalized gene expression dataset from a previous single-cell RNA-seq study [54]. E14.5, PN4–5 d, and PN14w, n = 8; PN4w, n = 4. B CD11c expression (reads per kilobase of exon model per million mapped reads) in the human brain at each stage of development. Each dot represents the average expression of each individual integrating multiple sites, and the solid line shows the approximate curve by local polynomial regression fitting. These data were generated using the gene expression dataset from BrainSpan (http://www.brainspan.org/, n = 42). C Clustering of snRNA-seq data in the PC. D Clustering of snRNA-seq data in the ACC. E Annotation according to known microglial marker genes in the PC. Microglia were identified as cluster 4. F Annotation according to known microglial marker genes in the ACC. Microglia were identified as cluster 8. G CD11c expression of microglia between control and ASD groups in the PC. H CD11c expression of microglia between control and ASD groups in the ACC. C–H Analyses of snRNA-seq data from a previous ASD study [55]. ACC, anterior cingulate cortex; AIF1, Allograft inflammatory factor 1; ASD, autism spectrum disorder; CX3CR1, CX3C motif chemokine receptor 1; PC, prefrontal cortex; UMAP, Uniform Manifold Approximation and Projection.