Fig. 3: Subgenomic isolation and B chromosome identification.

a Dotplot of co-orthologs genes and Ks within the P. australis genome. The color of the dot indicates the Ks of the gene pair. b Phylogenetic tree of genetic distances constructed based on single-copy homologous sequences. c Distribution of genes in the P. australis B chromosome in normal chromosomes. a Chromosomes. b Gene density. c Distribution of the coverage of multi-tissue mixed full-length transcriptome sequencing data. d - f. Distribution of leaf, aerial stem, and rhizome RNAseq data coverage, respectively. The blue connecting line in the figure indicates homology between the genes and those in the B chromosome with an E-value < 1e-10. d GO enrichment of genes expressed in the P. australis B chromosome. Genes with FPKM mean > 1 in chromosome B were retained for GO enrichment using clusterProfiler 2.0.