Fig. 6: Construction of the P. australis co-expression network.

a Score scatter plots for the PCA model with nine samples. b Heatmap of gene co-expression network module-tissue association. Each row corresponds to a module characteristic gene (eigengene), and each column corresponds to a specific tissue. Each cell contains the corresponding correlation value and P-value, and indicates the strength of the correlation according to the color. c A scatterplot of Gene Signifificance (GS) for weight vs. Module Membership (MM) in three module eigengene. There is a highly significant correlation between GS and MM in this module, illustrating that genes highly significantly associated with a trait are often also the most critical elements of modules associated with the trait. The red lines indicate the thresholds for |MM | > 0.8 and |GS | > 0.2, respectively. d Bubble diagram showing the results of GO enrichment of genes contained in the module eigengene. e Pattern map of P. australis (drawn by Congying Li). f Heatmap showing changes in module eigengene expression (FPKM) after row-scaling treatment. g Phragmites rhizoma Polysaccharide content in leaves, aerial stems, and rhizomes of P. australis. Polysaccharide mass (mg) = crude polysaccharide mass (mg) × polysaccharide concentration (%). Significance derived using a t-test, and different letters indicate significant differences (P < 0.05), n = 4.