Fig. 7: Lugen polysaccharide biosynthesis pathway and sugar transporter protein transcription factor identification. | Communications Biology

Fig. 7: Lugen polysaccharide biosynthesis pathway and sugar transporter protein transcription factor identification.

From: Telomere-to-telomere Phragmites australis reference genome assembly with a B chromosome provides insights into its evolution and polysaccharide biosynthesis

Fig. 7

a Gene expression analysis of genes involved in polysaccharide biosynthesis in “Lugen”. Different color blocks represent the expression levels of the encoded genes in different tissues, the squares from left to right correspond to leaves, aerial stems, and rhizomes, and the red font represents the gene family expansion in P. australis. Compound structure diagrams were obtained from EMBL-EBI (https://www.ebi.ac.uk/). b Prediction of cis-acting elements in the promoter region of the 12 identified PaSUC genes. A maximum likelihood phylogenetic tree (left) of 12 PaSUC family members was constructed using IQ-TREE (v 1.6.12) with 1,000 bootstrap replications. c Heatmap of sucrose transporters protein expression with transcription factors in Hub gene. The expression level of each gene is represented by log2 (FPKM), with redder colors indicating higher expression levels. d Hub genes co-expression network in the brown module. The connecting line indicates the co-expression relationship between TFs and PaSUC1.1 in hub genes. e Dual-luciferase assays demonstrate that PaR2R3-MYB transcriptionally activates PaSUC1.1. The data are presented as mean ± SD (n = 3), with error bars representing the SDs. Significant differences from the control were determined by Student’s t-test (P < 0.05).

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