Fig. 3: Depletion of OPTN in mice causes the disorder of mitophagy.
From: Fasting activates optineurin-mediated mitophagy in chondrocytes to protect against osteoarthritis
a Immunofluorescence staining of TOMM40 shows a decrease in the length-to-width ratio of mitochondria, suggesting an increase in fragmentation in chondrocytes of Optn-knockout (Optn−/−) mice. b Enhanced opening of mitochondrial membrane channels was observed in chondrocytes of Optn−/− mice as evidenced by a reduction in green fluorescence retention within the mitochondria. This indicates an increased degree of mPTP opening. c JC-1 staining shows an increased ratio of monomer/aggregate in chondrocytes of Optn−/− mice, indicating a decrease in mitochondrial membrane potential. d Decreased co-localization of MitoTracker and LC3 show suppressed autophagy in chondrocytes of Optn−/− mice. e–h Statistical analysis of quantified data shown in (a–d). Data show the mean ± SEM; Unpaired t-test, **p < 0.01, ***p < 0.001; n = 33, 11, 10, and 12 in (a–d), respectively.
