Fig. 1: An extensive chemical screening identifies five DVRs.
From: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
a Diagram showing the experimental conditions for the chemical screening to identify DVRs. Hydrated seeds were vernalized in 1.5-mL tubes and sown on cotton balls moistened with the test chemical (10 µM). After one week of cultivation, the plants were sprayed with luciferase substrate (luciferin in a solution containing Triton X-100), and luciferase activity was detected in planta using a CCD camera. b Diagram of the experimental strategy for characterizing DVRs. Hydrated seeds were vernalized in 1.5-mL tubes and sown on MS solid medium without (top) or with (bottom) DVRs (10 µM was used, except in the concentration dependency test of DVR06). After one week of cultivation, the plants were collected and subjected to RT-qPCR, GUS staining, ChIP-qPCR, RNA-seq, and ChIP-seq. For the flowering assays, the plants were transferred to soil (vermiculite and Metro-Mix) after one week of cultivation on solid MS medium with DVRs, then grown in the absence of DVRs.
