Fig. 2: Characterization of the devernalization inducers DVR02, DVR03, and DVR05.
From: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
a Chemical structures of DVR02, DVR03, and DVR05. Hydantoin-containing and spiro-like structures (upper left). Hydantoin-like and spiro-like structures are shown in red and blue, respectively. b Expression levels of endogenous FLC in vernalized plants (V) and in DVR02-, DVR03-, and DVR05-treated V plants. Error bars represent SD. Small circles represent data from individual plants. n = 3. *Significant difference from V plants (p < 0.05), as determined using Dunnett’s test. c Photographs of cotyledons and leaves of GUS-stained FLC::GUS V plants and DVR02-, DVR03-, and DVR05-treated FLC::GUS plants. Bars = 500 µm. d One week of DVR02, DVR03, and DVR05 treatment delays flowering time in V plants. Left: V: n = 10; DVR02: n = 11. Middle: V: n = 20; DVR03: n = 18. Right: V: n = 15; DVR05, n = 11. Error bars represent SD. Significant differences were determined using a two-tailed Student’s t-test (*p < 0.05). V, vernalized plants; DVR, DVR-treated V plants.
