Fig. 6: Low levels of H3K27me3 accumulate at the FLC locus in DVR06-treated V plants.
From: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
a Sites of PCR amplicons on the FLC locus. Upper: a gene model of FLC locus. Lower: the pattern of H3K27me3 accumulation on FLC locus in vernalized plants (V) based on ChIP-seq data (see Fig. 8). Promoter and 3′-untranslated region (UTR): black; intron: white; exon: gray. Red bars indicate the regions amplified by qPCR. b, c Accumulation levels of H3K27me3 (b) and H3K4me3 (c) in NV (non-vernalized), V (vernalized), and DVR06-treated V plants. The P1 region is located in the nucleation region. The ratios of ChIP to input DNA (% input) in each region were normalized using the value of % input for the positive control STM. Three independent experiments were performed. Error bars represent SD. Different letters indicate significant differences (p < 0.05), as determined using a one-way ANOVA followed by a Tukey-Kramer test. V, vernalized plants; NV, non-vernalized plants; DVR, DVR-treated V plants.
