Fig. 4: Temperature was the most important factor shaping intestinal microbial structure.

a Variance partitioning analysis was used to quantify the relative contributions made by environmental parameters and geographic distance to microbial structure of IM. b Structural equation modeling was conducted to partition the direct and indirect effects of multiple factors on microbial diversity and community structures. c Enterotype distribution at different ambient temperature. d Relationship between temperature and alpha diversity. As temperature rising, the Chao1 index and Shannon index increased. e Spearman’s correlation analysis of temperature and the three keystone genera.