Fig. 5: Experimental warming altered shrimp enterotypes distribution and regulating antimicrobial peptide expressions. | Communications Biology

Fig. 5: Experimental warming altered shrimp enterotypes distribution and regulating antimicrobial peptide expressions.

From: Warming-driven migration of enterotypes mediates host health and disease statuses in ectotherm Litopenaeus vannamei

Fig. 5

a Schematic representation of warming experiment combined with multiomics analysis and RNAi strategy. b Relative abundance of keystone genera at different temperature. c The Ratio of Vibrio and Candidatus Bacilloplasma decreased as ambient temperature raised. d As temperature increased, the Chao1 index and Shannon index tended to be higher. e MENA demonstrated that as temperature raised, the complexity and stability of species interaction network decreased. f PCoA showed that the microbial structure of different temperature was distinctive from each other (PerMANOVA, P = 0.008). g POD values for WFS and HPNS varied significantly at different ambient temperature. The above results were consistent with the results from the global data analysis, which confirmed that temperature exerted a profound influence on the microbial composition and structure. h A transcriptome analysis for shrimp intestine in different temperature was conducted to further explore how ambient warming alter IM composition. PCoA showed that samples from different temperature groups were separated (PerMANOVA, P = 0.044). i Alteration of keystone genera after silencing the expression of Pen2, Pen3, Pen4, Alf1, Alf2, Alf3 and Alf4 with RNAi strategy. The variation of keystone genera was consistent with the antimicrobial peptide expression status under different ambient temperature.

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