Fig. 7: Sofalcone suppressed the expression of CncC and Maf after exposure to insecticides.

Relative expression of transcription factors CncC (A) and Maf (B) when exposed to insecticides in the presence or absence of sofalcone was determined by RT-qPCR. The 2−ΔΔCT method was used for the quantification of the gene expression level. The cycle threshold (Ct) values for the genes were normalized to the Ct values of GAPDH and β-Actin. Data are the mean ± SD of five independent assays. The data were analyzed using Tukey’s HSD. Letters a, b and c denote significant differences at p < 0.05. n = 5.