Fig. 7: Low excitability of cerebellar Purkinje neurons of 2HIT-susceptible mice. | Communications Biology

Fig. 7: Low excitability of cerebellar Purkinje neurons of 2HIT-susceptible mice.

From: Maternal immune activation followed by peripubertal stress combinedly produce reactive microglia and confine cerebellar cognition

Fig. 7

a Representative action potentials of male cerebellar Purkinje cells, evoked by 400 pA and 500 ms step-pulse current injection. b Firing frequency of male mice of Control, RSDS-susceptible (RSDS), MIA, 2HIT-susceptible (2HIT), and 2HIT after microglia replacement (2HIT+rMG) Purkinje cells in response to gradually increased depolarization pulses from 0 to 550 pA. Two-way ANOVA group × injected current, interaction: F(40, 1364) = 1.00, p = 0.4765; main effect of group: F(4, 1364) = 24.3, p < 0.0001. *p < 0.05, multiple comparisons using the Bonferroni method. c Drawings of action potential waveform (left, a representative response to 400 pA pulse; right, single action potential and parameters (f-m). d-o Purkinje cell action potential properties, including series resistance (d), input resistance (e), basement potential of action potential (f), peak voltage of action potential (g), action potential threshold (h), amplitude of action potential (i), full width of half maximum (FWHM) (j), 10–90% rise time (k), fast afterhyperpolarization of the single action potential (fAHP) (l), slow afterhyperpolarization following action potential burst (sAHP) by 400 pA pulse (m), rheobase (n), and resting potential (o) are shown. All action potentials were measured in the initial action potential when evoked by the minimum depolarization pulses with a 50-pA step. The input resistance was measured as the current during 80 ms and 5 mV hyperpolarization pulse. The resting potential was measured as the voltage without current injection. *p < 0.05, one-way ANOVA with multiple comparisons using the Tukey-Kramer method. p Representative action potentials of female cerebellar Purkinje cells. q Firing frequency of female mice of Control, 2HIT-susceptible (2HIT sus), 2HIT-resilient (2HIT res), and 2HIT+rMG Purkinje cells in response to gradually increased depolarization pulses from 0 to 550 pA. Two-way ANOVA group × injected current, interaction: F(30, 990) = 1.08, p = 0.3526; main effect of group: F(3, 990) = 15.47, p < 0.0001. *p < 0.001, multiple comparisons using the Bonferroni method. r-ac Purkinje cell action potential properties are shown. *p < 0.05, one-way ANOVA with multiple comparisons using the Tukey-Kramer method. All data are represented as mean ± SEM.

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